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KMID : 0380020080230020183
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Korean Journal of Biotechnology and Bioengineering
2008 Volume.23 No. 2 p.183 ~ p.185
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Removal of Residual Toxic Phytohormone in Suspension Cultures of Panax Vietnamensis
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Lee Seung-Ho
Kim Nam-Hyuck
Quang Le Bach
Luong Hoang Van
Kim Ki-Hyun
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Abstract
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Studies were made to remove the toxic phytohormone, 2,4-D, in suspension cultures of Panax vietnamensis. Cells grown in normal MS medium with 2,4-D were inoculated and grown in the MS medium without hormone. Not a big difference was observed in growth characteristics between media with and without 2,4-D. The 2,4-D in the culture, however, was completely removed. During the culture, the residual 2,4-D was consumed rapidly at the early growth stage. The intra-cellular 2,4-D was consumed first and the 2,4-D in the medium was used afterward.
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KEYWORD
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Panax vietnamensis, 2, 4-D, toxic phytohormone, suspension culture
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